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BIO 482L/582L
Recombinant DNA Lab
Course Syllabus
Dr. Gary Stuart
Rm S233,
x-7898
gstuart@indstate.edu
http://mama.indstate.edu/users/stuart
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Link to On-line Course Materials:
- BIO482/582L
I. Construct Design
Jan. 14 - Project
Overview/Development
Jan. 21 - Sequence
Editing and Design
II. Vector Isolation
Jan. 28 - 1.1 Restriction Digest
Feb. 4 - 1.2 Preparative Agarose Gels
Feb. 11* - 1.3 Spin Column DNA
Purification
Feb. 18 - 1.4 Fluorometric
DNA Measurement
III. PCR Construction of Inserts
Feb. 25,4 - 2.1 Isolation
of Genomic DNA
Mar. 4 - 2.2 PCR Amplification
Mar. 11 - Spring
Break, no lab
Mar. 19 - 2.3 PCR Fragment Recovery
Mar. 25 - 2.4 Fragment Digest
IV. Ligation and Transformation
Apr. 1* - 3.1 PCR Fragment/Vector
Ligation
Apr. 8 - 3.2 Transformation &
Plating
Apr. 15 - 3.3 Plasmid Minipreps
(Th,F)
Apr. 22 - 3.4 Restriction Analysis
Apr. 29 - 3.5 Analytical Agarose Gels
May. 4* - Notebooks Due for Final
Examination
* Notebooks/Assignments
Due
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