YAC transfers
YAC transfers allow...
- complete promoters
- large genes
- entire gene clusters
Example1 -- human beta-globin in mice
- correct tissue specific and developmental expression
Example2 -- entire immunoglobulin loci
- light kappa, and heavy chain (midget loci)
- mice made with human genes bred to mice lacking mice genes (knockout with a few human genes)
- used to make human monoclonals
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