A. tumefaciens

3

• A phytopathogen: makes tumors in plants

• Tumors result from DNA transfer, integration

• Tumor inducing DNA: Ti plasmid

  • ori, T-DNA, vir, opine catabolism

• Transferred DNA (T-DNA) 12-24 kb

  • makes auxins, cytokinens, opines

• Wound molecules induce vir region of Ti

• Vir genes

  • 35kb region of Ti required for transfer, integration
  • not part of T-DNA

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