Bt toxin in water and underfoot
Mosquitos a difficult target
- Bt spores sink away from surface larvae in water
- alternative: clone into photosynthetic cyanobacteria which must proliferate near surface of water
- Asticcacaulis excentricus
- gram negative bacterium
- does not sink in water!
- Easy to grow on simple media
What about root (underground) pests!
- Clone into P. fluorescens (diazotroph)
- used disabled Tn5 sequences for homologous x-over
- very stable (not plasmid based)