Transcription: The Basics
RNA pol enzyme is processive
must bind tightly, but not too tightly
Movement like cytoskeletal motor kinesin
fluorescent bead covalently linked to one end
Allows determination of transcription rate
trap bead by laser beam & measure force
force ɮX that of myosin interacting with actin
Energy comes from NTP precursor hydrolysis
RNA pol may pause at some sites (Proof reading)