Thermus aquaticus were grown in a defined mineral salts medium containing the following components:
0.3% glutamic acid
0.1 mg/liter biotin
0.1 mg/liter thiamin
0.05 mg/liter nicotinic acid
100 mg/liter nitrilotriacetic acid
60 mg/liter CaSO4.2H2O
100 mg/liter Mg2SO4.7H2O
8 mg/liter NaCl
103 mg/liter KNO3
689 mg/liter NaNO3
5 mg/liter ZnSO4
5 mg/liter H3BO3
0.16 mg/liter CuSO4
0.25 mg/liter NaMoO4.2H2O
0.4 mg/liter CoCl2
0.28 mg/liter FeCl3
22 mg/liter MnSO4.H2O
110 mg/liter Na2HPO4
Adjust pH to 8.0 with NaOH
Reference:
Chien, A, Edgar, D.B. and Trela, J.M. 1976. Deoxyribonucleic Acid Polymerase from the Extreme Thermophile Thermus aquaticus. Journal of Bacteriology 127(3):1550-1557.